Clemson University is offering a service to determine resistance to a series of fungicides on your farm. See article below.
First results of 2013 monitoring for fungicide
resistance in the gray mold pathogen Botrytis cinerea
Dolores Fernández-Ortuño and Guido
Schnabel, Clemson University
Gray mold caused by the fungus Botrytis
cinerea is probably the most economically important disease of
strawberry worldwide. Infection of strawberry flowers is caused by spores
and results in fruit decay. Fruit infections begin as
small, firm, light brown lesions that enlarge quickly and fruit become covered
with a gray fuzzy mass of spores followed by a soft rot. Gray mold disease can
easily spread during periods of rainy and cool weather, heavy dews, or high
relative humidity.
Chemical control of
gray mold is essential to prevent fruit decay before and after harvest but resistance in B. cinerea to key fungicides is
emerging. Therefore we started an extension program at Clemson University, which
provides farm-specific fungicide resistance profiles. In our laboratory,
fungicide sensitivity assays are performed that allow the distinction of sensitive
from resistant isolates. During the 2011/2012 growing season we collected gray
mold from commercial fields in Arkansas, Florida, Georgia, Kansas, Maryland,
North Carolina, South Carolina, and Virginia. From each field we collected
spores from ten berries (called a sample) and confirmed that the gray mold
fungus was resistant to multiple chemical classes. The majority of the samples
analyzed contained fungus that was resistant to Topsin M and Pristine. Half of
the samples had a significant portion of fungus that was resistant to Scala and
Elevate fungicides. Resistance to Rovral and Switch was rare (Figure 1).
This season we are providing the same service to
strawberry growers and so far, we received samples from Georgia, Maryland,
North Carolina, South Carolina, and Virginia. Fungicide resistance is still
present and virtually all samples are resistant to Topsin M. In contrast to
last year, however, resistance to Pristine has not been found that often. Only
every 5th sample on average is resistant to Pristine. Rovral and
Switch remain to be great options against gray mold disease but remember that Rovral
is restricted to one application prior to bloom (Figure 1).
If you are are a strawberry or
blackberry grower and you are interested in getting your farm-specific
resistance profile to identify ineffective fungicides, send us around 40 dead
strawberry (or blackberry) flowers or collect spores from newly infected, decaying
fruit with a cotton swab. We need about 10 to 15 of those swabs (each from a
different fruit and each fruit from plants far enough apart to represent an
acre or so). Make sure that you only collect the fungus spores, do not touch
the fruit (Figure 2). Mail the
flowers or the swabs to Guido Schnabel, Clemson University, 114 Long Hall, Clemson,
SC 29634 and tell us the origin of the sample, your name, and e-mail so that we
can send you the report electronically. Upon receipt, we need about 3 (for
cotton swabs) to 5 (for flowers) working days to get a report to you outlining
farm specific gray mold management recommendations.
Figure 1. Percent of
samples with resistance to fungicide (red) collected from 8 states.
Figure 2. How to collect
spores from strawberries affected with gray mold using cotton swabs.
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