Why do we need fingerprints?
- Can sort mix ups in research and grower fields and nurseries
- Distinguish between closely related varieties
- Potential patent protection (not used yet for Rubus)
- Determine how diverse germplasm is in a breeding program
How do we produce fingerprints?
- Collect young leaf samples in spring or summer
- Extract DNA from leaves, and tag each plant using 6 DNA markers that are specific to red raspberry (Bassil et al.)
- Each genotype/variety/cultivar will have a unique profile or "fingerprint" for the 6 DNA markers
- The "fingerprint" of each sample is matched to a reference profile in our database, to ensure it is true to type (actually the genotype/cultivar/variety which it is labelled as).
This is a slide from one of the presentations. The first picture shows some of the leaf tissue, mortar and pestle (old fashion way to grind the tissue), the second picture is of extracted DNA in test tubes being prepared for analyses. The 3rd image is of a PCR printout, and the green bands and blue bands represent the DNA fragments, separated by size. Green and blue are two different markers, which are analyzed simultaneously.
In addition, through some modifications of the established protocol for red raspberry, we were able to determine that we could use these same markers for black raspberry and blackberry.
Bassil NV, Nyberg A, Hummer KE, Graham J, Dosset M, Finn CE. A Universal Fingerprinting Set for Red Raspberry. Proc. Xth Intl. Rubus and Ribes Symp. Acta Hort. 946. ISHS: 2012.